Bio Layer Interferometry

Bio-Layer Interferometry

Bio Layer Interferometry uses optical interferometry as means of detection. The technology uses light waves to infer the thickness of bio layer(s) on the sensor.

FortéBio’s BLI Octet systems offer real-time biomolecular interactions analysis in micro-volume samples, characterizing binding affinity, binding kinetics, and concentration.

Bio-Layer Interferometry (BLI) is a label-free technology for measuring biomolecular interactions. It is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on the biosensor tip, and an internal reference layer. Only molecules binding to or dissociating from the biosensor can shift the interference pattern and generate a response profile on the FortéBio Octet® System. Unbound molecules, changes in the refractive index of the surrounding medium, or changes in flow rate do not affect the interference pattern. This is a unique characteristic of BLI and extends its capability to perform in crude samples used in applications for protein: protein binding, quantitation, affinity, and kinetics. We have a range of solutions available from simple benchtop solutions, through to high throughput systems which can monitor up to 96 sensors in parallel.

BLI systems have a number of advantages including:

  • No microfluidic components: measure the samples directly in microplates
  • Up to 96 simultaneous measurements can be done on unpurified samples such as cell culture supernatants, lysates or complex mixtures in routine high throughput
  • High-throughput of crude samples is particularly useful for epitope binning, quantification, off-rate ranking or large screening assays where sample purification is not possible for efficient workflows
  • Large number of different biosensors available giving a broad range of surface chemistries to design experiments with maximum flexibility
BLI sensor
BLI sensor