LigandTracer offers a straightforward and reproducible method for detecting protein interactions on living cells over minutes, hours, or even days.
The use of living cells takes you one step closer to in vivo situations and is helpful for understanding biological processes, such as the uptake of drugs and the binding of imaging agents, as well as how cells integrate environmental clues into their signaling. The instrument is particularly suited to follow protein binding to cell surface receptors, and allows the measurement of on- and off-rates as well as affinities, without the need of washes or counting cells. The highly flexible nature of the technology enables a large number of additional applications, such as the measurement of cell-cell, protein-bacteria and protein-protein interactions.
Experiments are performed in disposable cell dishes and cells or liquid do not come in contact with any part of the instrument, resulting in a maintenance free system. LigandTracer can measure at room temperature, +4 °C, and +37 °C, making it possible to study the thermodynamics of interactions as well as cell behavior. The computer delivered with the instrument contains pre-installed software packages for assay setup (LigandTracer Control), support (LigandTracer Learning), and evaluation (TraceDrawer), all designed to be intuitive and meet the user’s needs.
More info about the LigandTracer on the website of Ridgeview.