Light scattering

Wyatt Technology Corp., based in Santa Barbara, CA, is the leading provider of analytical light scattering instrumentation for macromolecular characterization. While Wyatt detectors are most often used in combination with chromatography for determining absolute molar mass and size of macromolecules and particles in solution, the Calypso™ system is a novel application of light scattering, characterizing macromolecular interactions by means of Composition-Gradient Multi-Angle static Light Scattering (CG-MALS). CG-MALS measurements require no labeling or immobilization, for true free-solution, unmodified equilibrium and kinetic measurements to determine stoichiometry, binding affinity and kinetic rates. CG-MALS also characterizes non-specific interactions general solution-dependent attraction or repulsion between molecules that affect stability, aggregation, crystallization and purification.