Focal Molography

Focal Molography uses a laser beam to detect biomolecules bound to the active line on the surface which is diffracted into a focal point below the chip and is measured.

A mologram is a biological diffractive element fabricated using biomolecules and nanolithography approaches.

molecules + hologram = mologram

Focal molography combines state-of-the-art lithography, molecular self-assembly, and optical technology into one powerful technique. The technique allows biospecific molecular binding to be observed by cleverly exploiting the weak diffraction of light by a coherent pattern of molecules (or nanoparticles). Molecules that bind nonspecifically to the sensor surface contribute only incoherently to the sensor signal, and the coherent signal from analyte molecules on the sensor surface is much stronger than the signal from molecules that contribute incoherently. The focal molography method has two unique advantages in comparison with existing molecular sensing methods: (i) it eliminates the effect of nonbiospecific binding, which is the limiting factor in all current state-of-the-art molecular sensing devices, and (ii) it has a very high sensitivity that can be amplified by increasing the size of the sensor.

Mologram 1 Mologram 2
Detection set-up and chip set-up

More information at the website op lino Biotech AG.